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Mouse CD200 ELISA Kit PicoKine®, Carrier-free

Mouse CD200 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd200 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>76</td><td>78</td><td>75</td><td>70</td><td>74</td><td>2.94</td><td>3.9%</td></tr><tr><td>Sample 2</td><td>242</td><td>250</td><td>227</td><td>231</td><td>237</td><td>9.06</td><td>3.8%</td></tr><tr><td>Sample 3</td><td>1012</td><td>965</td><td>929</td><td>851</td><td>939</td><td>58.84</td><td>6.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

OX-2 membrane glycoprotein;MRC OX-2 antigen;CD200;Cd200;Mox2;

Gene Name

OX-2 membrane glycoprotein

Gene ID

17470

UniProt

O54901

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q31-G232

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues (By similarity). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

31256 MW

Specificity

Natural and recombinant mouse CD200

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Protein Name

OX-2 membrane glycoprotein

Frequently Asked Questions

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