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Mouse Sclerostin/SOST ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1179-carrier-free

Size: 96 Well With Removable Strips

Mouse Sclerostin/SOST ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Sost in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Sclerostin is a protein that in humans is encoded by the SOST gene. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>30</td><td>31</td><td>32</td><td>30</td><td>30</td><td>0.82</td><td>2.7%</td></tr><tr><td>Sample 2</td><td>190</td><td>179</td><td>179</td><td>181</td><td>182</td><td>4.54</td><td>2.4%</td></tr><tr><td>Sample 3</td><td>438</td><td>503</td><td>469</td><td>495</td><td>476</td><td>25.41</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Sclerostin;Sost;

Gene Name

Sclerostin

Gene ID

74499

UniProt

Q99P68

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-Y211

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

23443 MW

Specificity

Natural and recombinant mouse Sclerostin

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Belongs to the sclerostin family.

Protein Name

Sclerostin

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