Mouse TNFRSF9/4-1BB ELISA Kit PicoKine®, Carrier-free

Mouse TNFRSF9/4-1BB ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>95</td><td>83</td><td>93</td><td>86</td><td>89</td><td>4.91</td><td>5.5%</td></tr><tr><td>Sample 2</td><td>293</td><td>265</td><td>248</td><td>285</td><td>272</td><td>17.55</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>930</td><td>876</td><td>954</td><td>952</td><td>928</td><td>31.46</td><td>3.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Tumor necrosis factor receptor superfamily member 9;4-1BB ligand receptor;T-cell antigen 4-1BB;CD137;Tnfrsf9;Cd137, Ila, Ly63;

Gene Name

Tumor necrosis factor receptor superfamily member 9

Gene ID

21942

UniProt

P20334

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V24-L187

Tissue Specificity

Expressed on the surface of activated T-cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)