Mouse IL15RA ELISA Kit PicoKine®, Carrier-free

Mouse IL15RA ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il15ra in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>181</td><td>196</td><td>202</td><td>196</td><td>193</td><td>7.75</td><td>4%</td></tr><tr><td>Sample 2</td><td>1121</td><td>1352</td><td>1324</td><td>1168</td><td>1241</td><td>98.66</td><td>7.9%</td></tr><tr><td>Sample 3</td><td>6129</td><td>5379</td><td>6344</td><td>6290</td><td>6035</td><td>387.19</td><td>6.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-15 receptor subunit alpha;IL-15 receptor subunit alpha;IL-15R-alpha;IL-15RA;CD215;Soluble interleukin-15 receptor subunit alpha;sIL-15 receptor subunit alpha;sIL-15R-alpha;sIL-15RA;Il15ra;

Gene Name

Interleukin-15 receptor subunit alpha

Gene ID

16169

UniProt

Q60819

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G33-K205

Tissue Specificity

Widely expressed. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

125 pg/ml - 8,000 pg/ml

Sensitivity

<10 pg/ml

Function

High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Signal transduction involves SYK (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)