Mouse CCL8/MCP-2 ELISA Kit PicoKine®, Carrier-free

Mouse CCL8/MCP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>37</td><td>36</td><td>34</td><td>32</td><td>34</td><td>1.92</td><td>5.6%</td></tr><tr><td>Sample 2</td><td>190</td><td>188</td><td>192</td><td>174</td><td>186</td><td>7.07</td><td>3.8%</td></tr><tr><td>Sample 3</td><td>475</td><td>541</td><td>471</td><td>504</td><td>497</td><td>28.03</td><td>5.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-C motif chemokine 8;Monocyte chemoattractant protein 2;Monocyte chemotactic protein 2;MCP-2;Small-inducible cytokine A8;Ccl8;Mcp2, Scya8;

Gene Name

C-C motif chemokine 8

Gene ID

20307

UniProt

Q9Z121

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E20-P97

Applications

ELISA

Standard

1 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Chemotactic factor that attracts monocytes. This protein can bind heparin (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)