Mouse CCL6/C10 ELISA Kit PicoKine®, Carrier-free

Mouse CCL6/C10 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 6 (CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents.In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>33</td><td>33</td><td>30</td><td>31</td><td>31</td><td>1.29</td><td>4.1%</td></tr><tr><td>Sample 2</td><td>164</td><td>163</td><td>160</td><td>168</td><td>163</td><td>2.86</td><td>1.7%</td></tr><tr><td>Sample 3</td><td>462</td><td>418</td><td>416</td><td>460</td><td>439</td><td>22.02</td><td>5%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-C motif chemokine 6;Protein C10;Small-inducible cytokine A6;CCL6 (22-95);CCL6 (23-95);Ccl6;C10, Scya6;

Gene Name

C-C motif chemokine 6

Gene ID

20305

UniProt

P27784

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G22-A116

Tissue Specificity

Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

CCL6 (22-95) and CCL6 (23-95) are potent chemoattractants. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)