Human Galectin-9 / Gal 9 ELISA Kit PicoKine®, Carrier-free

Human Galectin-9 / Gal 9 ELISA Kit PicoKine® (96 Tests). Quantitate Human LGALS9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-9, also called HUAT or LGALS9A is a protein that in humans is encoded by the LGALS9 gene. This gene is mapped to 17q11.2. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>168</td><td>165</td><td>163</td><td>179</td><td>168</td><td>6.17</td><td>3.6%</td></tr><tr><td>Sample 2</td><td>731</td><td>656</td><td>726</td><td>619</td><td>683</td><td>47.37</td><td>6.9%</td></tr><tr><td>Sample 3</td><td>3580</td><td>3263</td><td>3243</td><td>3664</td><td>3437</td><td>187</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Galectin-9;Gal-9;Ecalectin;Tumor antigen HOM-HD-21;LGALS9;

Gene Name

Galectin-9

Gene ID

3965

UniProt

O00182

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-T355

Tissue Specificity

Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

93.8 pg/ml - 6,000 pg/ml

Sensitivity

<10 pg/ml

Function

Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)