Rat TGF-Beta 2 ELISA Kit PicoKine®, Carrier-free

Rat TGF-Beta 2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Tgfb2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>57</td><td>55</td><td>54</td><td>54</td><td>55</td><td>1.22</td><td>2.2%</td></tr><tr><td>Sample 2</td><td>207</td><td>189</td><td>219</td><td>204</td><td>204</td><td>10.68</td><td>5.2%</td></tr><tr><td>Sample 3</td><td>866</td><td>820</td><td>926</td><td>906</td><td>879</td><td>40.58</td><td>4.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Transforming growth factor beta-2;TGF-beta-2;Latency-associated peptide;LAP;Tgfb2;

Gene Name

Transforming growth factor beta-2

Gene ID

81809

UniProt

Q07257

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A303-S414

Tissue Specificity

Isoform TGF-beta2B expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)