Human Betacellulin/BTC ELISA Kit PicoKine®, Carrier-free

Human Betacellulin/BTC ELISA Kit PicoKine® (96 Tests). Quantitate Human BTC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>36</td><td>37</td><td>37</td><td>33</td><td>35</td><td>1.63</td><td>4.6%</td></tr><tr><td>Sample 2</td><td>220</td><td>251</td><td>215</td><td>249</td><td>233</td><td>16.36</td><td>7%</td></tr><tr><td>Sample 3</td><td>402</td><td>461</td><td>474</td><td>465</td><td>450</td><td>28.39</td><td>6.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Probetacellulin;Betacellulin;BTC;BTC;

Gene Name

Probetacellulin

Gene ID

685

UniProt

P35070

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D32-Y111

Tissue Specificity

Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)