Human Progranulin ELISA Kit PicoKine®, Carrier-free

Human Progranulin ELISA Kit PicoKine® (96 Tests). Quantitate Human GRN in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Granulin, also known as Proepithelin (PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>507</td><td>529</td><td>566</td><td>377</td><td>495</td><td>28.05</td><td>5.7%</td></tr><tr><td>Sample 2</td><td>1173</td><td>1005</td><td>874</td><td>1096</td><td>1037</td><td>69.89</td><td>6.7%</td></tr><tr><td>Sample 3</td><td>9591</td><td>8789</td><td>9119</td><td>5790</td><td>8322</td><td>566.75</td><td>6.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Granulins;Proepithelin;PEPI;Acrogranin;Glycoprotein of 88 Kda;GP88;Glycoprotein 88;Progranulin;Paragranulin;Granulin-1;Granulin G;Granulin-2;Granulin F;Granulin-3;Granulin B;Granulin-4;Granulin A;Granulin-5;Granulin C;Granulin-6;Granulin D;Granulin-7;Granulin E;GRN;

Gene Name

Granulins

Gene ID

2896

UniProt

P28799

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T18-L593

Tissue Specificity

In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<150 pg/ml

Function

Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)