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Human FSTL1/Tsc 36 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0965-carrier-free

Size: 96 Well With Removable Strips

Human FSTL1/Tsc 36 ELISA Kit PicoKine® (96 Tests). Quantitate Human FSTL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>591</td><td>609</td><td>629</td><td>635</td><td>616</td><td>17.34</td><td>2.8%</td></tr><tr><td>Sample 2</td><td>1511</td><td>1639</td><td>1624</td><td>1771</td><td>1636</td><td>92.19</td><td>5.6%</td></tr><tr><td>Sample 3</td><td>9939</td><td>8661</td><td>9621</td><td>8867</td><td>9272</td><td>525.36</td><td>5.6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Follistatin-related protein 1;Follistatin-like protein 1;FSTL1;FRP;

Gene Name

Follistatin-related protein 1

Gene ID

11167

UniProt

Q12841

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-I308

Tissue Specificity

Overexpressed in synovial tissues from rheumatoid arthritis. .

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin (By similarity). .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

34986 MW

Specificity

Natural and recombinant human FSTL1

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Sequence Similarities

Contains 2 EF-hand domains.

Protein Name

Follistatin-related protein 1

Price

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