Human IGFBP6 ELISA Kit PicoKine®, Carrier-free

Human IGFBP6 ELISA Kit PicoKine® (96 Tests). Quantitate Human IGFBP6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor-binding protein 6 (IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>162</td><td>158</td><td>152</td><td>159</td><td>157</td><td>3.63</td><td>2.3%</td></tr><tr><td>Sample 2</td><td>611</td><td>642</td><td>578</td><td>623</td><td>613</td><td>23.28</td><td>3.7%</td></tr><tr><td>Sample 3</td><td>2123</td><td>2028</td><td>2170</td><td>1875</td><td>2049</td><td>112.73</td><td>5.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Insulin-like growth factor-binding protein 6;IBP-6;IGF-binding protein 6;IGFBP-6;IGFBP6;IBP6;

Gene Name

Insulin-like growth factor-binding protein 6

Gene ID

3489

UniProt

P24592

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A25-G240

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)