Human EPCR ELISA Kit PicoKine®, Carrier-free

Human EPCR ELISA Kit PicoKine® (96 Tests). Quantitate Human PROCR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Endothelial protein C receptor (EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>678</td><td>818</td><td>815</td><td>809</td><td>780</td><td>58.97</td><td>7.5%</td></tr><tr><td>Sample 2</td><td>1520</td><td>1504</td><td>1453</td><td>1532</td><td>1502</td><td>30.11</td><td>2%</td></tr><tr><td>Sample 3</td><td>9734</td><td>8679</td><td>10303</td><td>9352</td><td>9517</td><td>590.41</td><td>6.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Endothelial protein C receptor;Activated protein C receptor;APC receptor;Endothelial cell protein C receptor;CD201;PROCR;EPCR;

Gene Name

Endothelial protein C receptor

Gene ID

10544

UniProt

Q9UNN8

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-S210

Tissue Specificity

Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)