Human Endothelin ELISA Kit PicoKine®, Carrier-free

Human Endothelin ELISA Kit PicoKine® (96 Tests). Quantitate Human Endothelin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>11</td><td>13</td><td>12</td><td>14</td><td>12</td><td>1.11</td><td>9.2%</td></tr><tr><td>Sample 2</td><td>51</td><td>49</td><td>47</td><td>47</td><td>48</td><td>1.65</td><td>3.4%</td></tr><tr><td>Sample 3</td><td>131</td><td>117</td><td>110</td><td>124</td><td>120</td><td>7.82</td><td>6.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Endothelin-1;Preproendothelin-1;PPET1;Endothelin-1;ET-1;Big endothelin-1;EDN1;

Gene Name

endothelin 1, endothelin 2, endothelin 3

Gene ID

1906, 1907, 1908

UniProt

P05305, P20800, P14138

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

A peptide reflecting the target protein in human. Sequence: C53-W73

Tissue Specificity

Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. .

Applications

ELISA

Standard

1 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

3.9 pg/ml - 250 pg/ml

Sensitivity

<0.5 pg/ml

Function

Endothelins are endothelium-derived vasoconstrictor peptides.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)