Human MyD88 ELISA Kit PicoKine®, Carrier-free

Human MyD88 ELISA Kit PicoKine® (96 Tests). Quantitate Human MYD88 in cell culture supernatants, cell lysates, bone tissue, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Myeloid differentiation primary response gene (88) (MYD88) is a protein that, in humans, is encoded by the MYD88 gene. By interspecific backcross mapping, the mouse MyD88 gene was localized to chromosome 9; the human homolog was mapped to 3p22-p21.3 The human ortholog MYD88 seems to function similarly to mice, however, available evidence suggests that MYD88 is dispensable for human resistance to common viral infections and to all but a few pyogenic bacterial infections, demonstrating a major difference between mouse and human immune responses.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>244</td><td>207</td><td>232</td><td>211</td><td>223</td><td>15.17</td><td>6.8%</td></tr><tr><td>Sample 2</td><td>985</td><td>915</td><td>1056</td><td>940</td><td>974</td><td>53.57</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>5638</td><td>5953</td><td>5999</td><td>5631</td><td>5805</td><td>171.54</td><td>2.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Myeloid differentiation primary response protein MyD88;MYD88;

Gene Name

Myeloid differentiation primary response protein MyD88

Gene ID

4615

UniProt

Q99836

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-P296

Tissue Specificity

Ubiquitous. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, bone tissue, serum and plasma (heparin, EDTA)

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)