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Human MIA ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0941-carrier-free

Size: 96 Well With Removable Strips

Human MIA ELISA Kit PicoKine® (96 Tests). Quantitate Human MIA in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain (1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>29</td><td>28</td><td>27</td><td>29</td><td>28</td><td>0.82</td><td>2.9%</td></tr><tr><td>Sample 2</td><td>172</td><td>166</td><td>171</td><td>170</td><td>169</td><td>2.27</td><td>1.3%</td></tr><tr><td>Sample 3</td><td>485</td><td>453</td><td>511</td><td>458</td><td>476</td><td>23.22</td><td>4.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Melanoma-derived growth regulatory protein;Melanoma inhibitory activity protein;MIA;

Gene Name

Melanoma-derived growth regulatory protein

Gene ID

8190

UniProt

Q16674

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G25-131Q

Tissue Specificity

All malignant melanoma cell lines tested and infrequently in glioma cell lines.

Applications

ELISA

Standard

1 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

14509 MW

Specificity

Natural and recombinant human MIA

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the MIA/OTOR family.

Protein Name

Melanoma-derived growth regulatory protein

Price

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