Human MIA ELISA Kit PicoKine®, Carrier-free

Human MIA ELISA Kit PicoKine® (96 Tests). Quantitate Human MIA in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain (1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>29</td><td>28</td><td>27</td><td>29</td><td>28</td><td>0.82</td><td>2.9%</td></tr><tr><td>Sample 2</td><td>172</td><td>166</td><td>171</td><td>170</td><td>169</td><td>2.27</td><td>1.3%</td></tr><tr><td>Sample 3</td><td>485</td><td>453</td><td>511</td><td>458</td><td>476</td><td>23.22</td><td>4.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Melanoma-derived growth regulatory protein;Melanoma inhibitory activity protein;MIA;

Gene Name

Melanoma-derived growth regulatory protein

Gene ID

8190

UniProt

Q16674

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G25-131Q

Tissue Specificity

All malignant melanoma cell lines tested and infrequently in glioma cell lines.

Applications

ELISA

Standard

1 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Function

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)