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Human ICAM-3 / CD50 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0940-carrier-free

Size: 96 Well With Removable Strips

Human ICAM-3 / CD50 ELISA Kit PicoKine® (96 Tests). Quantitate Human ICAM3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>378</td><td>319</td><td>323</td><td>361</td><td>345</td><td>25.02</td><td>7.2%</td></tr><tr><td>Sample 2</td><td>1191</td><td>1222</td><td>1181</td><td>1135</td><td>1182</td><td>31.18</td><td>2.6%</td></tr><tr><td>Sample 3</td><td>4088</td><td>4762</td><td>4153</td><td>4037</td><td>4260</td><td>292.73</td><td>6.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Intercellular adhesion molecule 3;ICAM-3;CDw50;ICAM-R;CD50;ICAM3;

Gene Name

Intercellular adhesion molecule 3

Gene ID

3385

UniProt

P32942

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q30-H485; homodimer

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

59541 MW

Specificity

Natural and recombinant human ICAM-3

Anticoagulant

heparin or EDTA

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the immunoglobulin superfamily. ICAM family.

Protein Name

Intercellular adhesion molecule 3

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