Human Furin ELISA Kit PicoKine®, Carrier-free

Human Furin ELISA Kit PicoKine® (96 Tests). Quantitate Human FURIN in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>417</td><td>420</td><td>397</td><td>384</td><td>404</td><td>14.77</td><td>3.6%</td></tr><tr><td>Sample 2</td><td>2751</td><td>2551</td><td>2738</td><td>2985</td><td>2756</td><td>153.96</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>8293</td><td>8993</td><td>7686</td><td>9246</td><td>8554</td><td>610.96</td><td>7.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Furin;3.4.21.75;Dibasic-processing enzyme;Paired basic amino acid residue-cleaving enzyme;PACE;FURIN;FUR, PACE, PCSK3;

Gene Name

Furin

Gene ID

5045

UniProt

P09958

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D108-E715

Tissue Specificity

Seems to be expressed ubiquitously.

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants and cell lysates.

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R)R consensus motif. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

86678 MW

Specificity

Natural and recombinant human Furin

Subcellular Location

Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.