Mouse CD25/IL-2sR Alpha ELISA Kit PicoKine®, Carrier-free

Mouse CD25/IL-2sR Alpha ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il2ra in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>143</td><td>134</td><td>137</td><td>152</td><td>141</td><td>6.87</td><td>4.8%</td></tr><tr><td>Sample 2</td><td>707</td><td>650</td><td>699</td><td>681</td><td>684</td><td>21.9</td><td>3.2%</td></tr><tr><td>Sample 3</td><td>2735</td><td>2916</td><td>2595</td><td>2589</td><td>2708</td><td>133.15</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-2 receptor subunit alpha;IL-2 receptor subunit alpha;IL-2-RA;IL-2R subunit alpha;IL2-RA;p55;CD25;Il2ra;Il2r;

Gene Name

Interleukin-2 receptor subunit alpha

Gene ID

16184

UniProt

P01590

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-K236

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

78 pg/ml - 5,000 pg/ml

Sensitivity

<5 pg/ml

Function

Receptor for interleukin-2.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)