Rat MCP-1 ELISA Kit PicoKine®, Carrier-free

Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>41</td><td>37</td><td>39</td><td>42</td><td>39</td><td>1.92</td><td>4.9%</td></tr><tr><td>Sample 2</td><td>257</td><td>231</td><td>216</td><td>228</td><td>233</td><td>14.94</td><td>6.4%</td></tr><tr><td>Sample 3</td><td>473</td><td>462</td><td>446</td><td>461</td><td>460</td><td>9.6</td><td>2%</td></tr></table>*number of samples for each test n=16.

C-C motif chemokine 2;Immediate-early serum-responsive protein JE;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;MCP-1;Small-inducible cytokine A2;CCL2;Je, Mcp1, Scya2;

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles. (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)