Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®, Carrier-free

Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human THBS1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2 (region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>2414</td><td>2383</td><td>2098</td><td>2136</td><td>2257</td><td>141.81</td><td>6.2%</td></tr><tr><td>Sample 2</td><td>13444</td><td>12251</td><td>11532</td><td>12803</td><td>12507</td><td>703.87</td><td>5.6%</td></tr><tr><td>Sample 3</td><td>38908</td><td>44108</td><td>38626</td><td>45595</td><td>41809</td><td>3088.95</td><td>7.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Thrombospondin-1;THBS1;TSP, TSP1;

Gene Name

Thrombospondin-1

Gene ID

7057

UniProt

P07996

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N19-P1170

Applications

ELISA

Standard

100 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

1.56 ng/ml - 100 ng/ml

Sensitivity

<50 pg/ml

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)