Human Endostatin ELISA Kit PicoKine®, Carrier-free

Human Endostatin ELISA Kit PicoKine® (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>247</td><td>238</td><td>232</td><td>204</td><td>230</td><td>16.06</td><td>6.9%</td></tr><tr><td>Sample 2</td><td>1523</td><td>1309</td><td>1362</td><td>1479</td><td>1418</td><td>86.25</td><td>6%</td></tr><tr><td>Sample 3</td><td>5144</td><td>5141</td><td>5851</td><td>5778</td><td>5478</td><td>336.99</td><td>6.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Collagen alpha-1 (XVIII) chain;Endostatin;COL18A1;

Gene Name

Collagen alpha-1(XVIII) chain

Gene ID

80781

UniProt

P39060

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H1154-S1335

Tissue Specificity

Present in multiple organs with highest levels in liver, lung and kidney.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)