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Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®, Carrier-free

Human IL-18 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>33</td><td>32</td><td>33</td><td>31</td><td>32</td><td>0.82</td><td>2.5%</td></tr><tr><td>Sample 2</td><td>247</td><td>212</td><td>221</td><td>232</td><td>228</td><td>13.05</td><td>5.7%</td></tr><tr><td>Sample 3</td><td>520</td><td>533</td><td>562</td><td>546</td><td>478</td><td>31.15</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-18;IL-18;Iboctadekin;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;IL18;IGIF, IL1F4;

Gene Name

Interleukin-18

Gene ID

3606

UniProt

Q14116

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Y33-D197

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<1 pg/ml

Function

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22326 MW

Specificity

Natural and recombinant human IL-18

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-1 family.

Protein Name

Interleukin-18

Frequently Asked Questions

More Discoveries

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