Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine®, Carrier-free

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine® (96 Tests). Quantitate Human TFPI in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TFPI (Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>57</td><td>57</td><td>66</td><td>64</td><td>61</td><td>4.06</td><td>6.6%</td></tr><tr><td>Sample 2</td><td>238</td><td>272</td><td>240</td><td>245</td><td>248</td><td>13.66</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>848</td><td>982</td><td>998</td><td>845</td><td>918</td><td>71.98</td><td>7.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Tissue factor pathway inhibitor;TFPI;Extrinsic pathway inhibitor;EPI;Lipoprotein-associated coagulation inhibitor;LACI;TFPI;LACI, TFPI1;

Gene Name

Tissue factor pathway inhibitor

Gene ID

7035

UniProt

P10646

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D29-K282

Tissue Specificity

Mostly in endothelial cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Inhibits factor X (X (a)) ly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)