Human NOV/CCN3 ELISA Kit PicoKine®, Carrier-free

Human NOV/CCN3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCN3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>66</td><td>61</td><td>66</td><td>62</td><td>63</td><td>2.27</td><td>3.6%</td></tr><tr><td>Sample 2</td><td>265</td><td>294</td><td>297</td><td>292</td><td>287</td><td>12.82</td><td>4.4%</td></tr><tr><td>Sample 3</td><td>820</td><td>835</td><td>750</td><td>802</td><td>801</td><td>32.08</td><td>4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Protein NOV homolog;NovH;CCN family member 3;Insulin-like growth factor-binding protein 9;IBP-9;IGF-binding protein 9;IGFBP-9;Nephroblastoma-overexpressed gene protein homolog;NOV;CCN3, IGFBP9, NOVH;

Gene Name

Protein NOV homolog

Gene ID

4856

UniProt

P48745

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T32-M357

Tissue Specificity

Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<2 pg/ml

Function

Immediate-early protein likely to play a role in cell growth regulation. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)