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Human Prostate-specific antigen ELISA kit (PSA/KLK3) PicoKine®, Carrier-free

Selected Variant

EK0816-carrier-free

Size: 96 Well With Removable Strips

Human Kallikrein 3/PSA/KLK3 ELISA Kit PicoKine® (96 Tests). Quantitate Human KLK3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Prostate-specific antigen (PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome (19q13).3

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>551</td><td>505</td><td>496</td><td>595</td><td>536</td><td>39.57</td><td>7.3%</td></tr><tr><td>Sample 2</td><td>2528</td><td>2531</td><td>2745</td><td>2357</td><td>2540</td><td>137.6</td><td>5.4%</td></tr><tr><td>Sample 3</td><td>8207</td><td>9826</td><td>9283</td><td>9589</td><td>9226</td><td>619.14</td><td>6.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Prostate-specific antigen;PSA;3.4.21.77;Gamma-seminoprotein;Seminin;Kallikrein-3;P-30 antigen;Semenogelase;KLK3;APS;

Gene Name

Prostate-specific antigen

Gene ID

354

UniProt

P07288

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-P261

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

28741 MW

Specificity

Natural and recombinant human Kallikrein 3

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein Name

Prostate-specific antigen

Price

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