Mouse IL-1RA/IL1RN ELISA Kit PicoKine®, Carrier-free

Mouse IL-1RA/IL1RN ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il1rn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization. IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>99</td><td>89</td><td>98</td><td>95</td><td>95</td><td>3.89</td><td>4%</td></tr><tr><td>Sample 2</td><td>586</td><td>555</td><td>523</td><td>545</td><td>552</td><td>22.66</td><td>4.1%</td></tr><tr><td>Sample 3</td><td>1056</td><td>1070</td><td>1157</td><td>1068</td><td>1087</td><td>40.33</td><td>3.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-1 receptor antagonist protein;IL-1RN;IL-1ra;IRAP;IL1 inhibitor;Il1rn;Il-1ra;

Gene Name

Interleukin-1 receptor antagonist protein

Gene ID

16181

UniProt

P25085

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R27-Q178

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)