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Mouse IL-7/Interleukin-7 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0780-carrier-free

Size: 96 Well With Removable Strips

Mouse IL-7/Interleukin-7 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il7 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 7 (IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7 (IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17 (T (H)17) cells in experimental autoimmune encephalomyelitis (EAE).2

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>30</td><td>31</td><td>31</td><td>33</td><td>31</td><td>1.08</td><td>3.4%</td></tr><tr><td>Sample 2</td><td>185</td><td>168</td><td>189</td><td>181</td><td>180</td><td>7.88</td><td>4.3%</td></tr><tr><td>Sample 3</td><td>388</td><td>464</td><td>422</td><td>442</td><td>429</td><td>27.94</td><td>6.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-7;IL-7;Il7;Il-7;

Gene Name

Interleukin-7

Gene ID

16196

UniProt

P10168

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E26-I154

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<1 pg/ml

Function

Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17727 MW

Specificity

Natural and recombinant mouse IL-7

Anticoagulant

EDTA

Subcellular Location

Secreted.

Protein Name

Interleukin-7

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