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Human IL-7/Interleukin-7 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0779-carrier-free

Size: 96 Well With Removable Strips

Human IL-7/Interleukin-7 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL7 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7 (IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V (D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>17</td><td>18</td><td>18</td><td>18</td><td>17</td><td>0.43</td><td>2.5%</td></tr><tr><td>Sample 2</td><td>81</td><td>89</td><td>83</td><td>75</td><td>82</td><td>5</td><td>6%</td></tr><tr><td>Sample 3</td><td>209</td><td>226</td><td>204</td><td>221</td><td>215</td><td>8.86</td><td>4.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-7 ;IL-7 ;

Gene Name

Interleukin-7

Gene ID

3574

UniProt

P13232

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D26-H177

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (EDTA).

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<1 pg/ml

Function

Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20217 MW

Specificity

Natural and recombinant human IL-7

Anticoagulant

EDTA

Subcellular Location

Secreted .

Protein Name

Interleukin-7

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