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Mouse Decorin ELISA Kit PicoKine®, Carrier-free

Mouse Decorin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Dcn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>68</td><td>67</td><td>60</td><td>65</td><td>65</td><td>3.08</td><td>4.7%</td></tr><tr><td>Sample 2</td><td>227</td><td>213</td><td>209</td><td>242</td><td>222</td><td>12.96</td><td>5.8%</td></tr><tr><td>Sample 3</td><td>930</td><td>785</td><td>877</td><td>787</td><td>844</td><td>61.66</td><td>7.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Decorin;Bone proteoglycan II;PG-S2;PG40;Dcn;

Gene Name

Decorin

Gene ID

13179

UniProt

P28654

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D31-K354

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

May affect the rate of fibrils formation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

39809 MW

Specificity

Natural and recombinant human Decorin

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sequence Similarities

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Protein Name

Decorin

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