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Rat CD86/B7-2 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0712-carrier-free

Size: 96 Well With Removable Strips

Rat CD86/B7-2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cd86 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>179</td><td>159</td><td>158</td><td>184</td><td>170</td><td>11.64</td><td>6.8%</td></tr><tr><td>Sample 2</td><td>612</td><td>691</td><td>664</td><td>617</td><td>646</td><td>32.96</td><td>5.1%</td></tr><tr><td>Sample 3</td><td>1878</td><td>1659</td><td>1901</td><td>1936</td><td>1843</td><td>108.5</td><td>5.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

B7-2 ;Cd86 ;

Gene Name

CD86 molecule

Gene ID

56822

UniProt

Q62810

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V29-I250

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17153 MW

Specificity

Natural and recombinant rat CD86

Protein Name

T-lymphocyte activation antigen CD86

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