Mouse CD14 ELISA Kit PicoKine®, Carrier-free

Mouse CD14 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1809</td><td>1708</td><td>1610</td><td>1761</td><td>1722</td><td>73.87</td><td>4.2%</td></tr><tr><td>Sample 2</td><td>4760</td><td>4159</td><td>4699</td><td>4655</td><td>4568</td><td>239.2</td><td>5.2%</td></tr><tr><td>Sample 3</td><td>24340</td><td>25258</td><td>25930</td><td>26104</td><td>25408</td><td>692.82</td><td>2.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Monocyte differentiation antigen CD14;Myeloid cell-specific leucine-rich glycoprotein;CD14;Cd14;

Gene Name

Monocyte differentiation antigen CD14

Gene ID

12475

UniProt

P10810

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-P345

Applications

ELISA

Standard

50 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

0.78 ng/ml - 50 ng/ml

Sensitivity

<10 pg/ml

Function

In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)