Mouse Cathepsin D ELISA Kit PicoKine®, Carrier-free

Mouse Cathepsin D ELISA Kit PicoKine® (96 Tests). Quantitate Mouse CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>844</td><td>838</td><td>787</td><td>752</td><td>805</td><td>37.89</td><td>4.7%</td></tr><tr><td>Sample 2</td><td>1778</td><td>2039</td><td>1841</td><td>1903</td><td>1890</td><td>96.58</td><td>5.1%</td></tr><tr><td>Sample 3</td><td>7768</td><td>8873</td><td>9192</td><td>9277</td><td>8777</td><td>601.98</td><td>6.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Cathepsin D;3.4.23.5;CTSD;

Gene Name

Cathepsin D

Gene ID

13033

UniProt

Q3UCD9

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I21-L410

Applications

ELISA

Standard

20 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Function

Acid protease active in intracellular protein breakdown.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)