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Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine®, Carrier-free

Human TSP2 ELISA Kit PicoKine® (96 Tests). Quantitate Human THBS2 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Thrombospondin-2 (TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>234</td><td>245</td><td>217</td><td>210</td><td>226</td><td>13.79</td><td>6.1%</td></tr><tr><td>Sample 2</td><td>1702</td><td>1693</td><td>1719</td><td>1783</td><td>1724</td><td>35.18</td><td>2%</td></tr><tr><td>Sample 3</td><td>6045</td><td>5875</td><td>6096</td><td>5562</td><td>5894</td><td>208.67</td><td>3.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Thrombospondin-2;THBS2;TSP2;

Gene Name

Thrombospondin-2

Gene ID

7058

UniProt

P35442

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G19-I1172

Tissue Specificity

High expression in invertebral disk tissue. .

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Detection Range

625 pg/ml - 40,000 pg/ml

Sensitivity

<10 pg/ml

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

129991 MW

Specificity

Natural and recombinant human TSP2

Anticoagulant

heparin or EDTA

Sequence Similarities

Belongs to the thrombospondin family.

Protein Name

Thrombospondin-2

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