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Human Prolactin ELISA Kit PicoKine®, Carrier-free

Human Prolactin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRL in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis,angiogenesis and is involved in the regulation of blood clotting through several pathways.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>278</td><td>266</td><td>323</td><td>299</td><td>291</td><td>21.68</td><td>7.4%</td></tr><tr><td>Sample 2</td><td>1989</td><td>1892</td><td>1944</td><td>1862</td><td>1921</td><td>48.66</td><td>2.5%</td></tr><tr><td>Sample 3</td><td>4706</td><td>4695</td><td>5058</td><td>4926</td><td>4846</td><td>153.08</td><td>3.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Prolactin;PRL;PRL;

Gene Name

Prolactin

Gene ID

5617

UniProt

P01236

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L29-C227

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, citrate).

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<10 pg/ml

Function

Prolactin acts primarily on the mammary gland by promoting lactation.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

25876 MW

Specificity

Natural and recombinant human Prolactin

Anticoagulant

heparin or citrate

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the somatotropin/prolactin family.

Protein Name

Prolactin

Curated Selection

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