Mouse CD30L ELISA Kit PicoKine®, Carrier-free

Mouse CD30L ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfsf8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The human CD30L (CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154 (CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this"reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>35</td><td>30</td><td>34</td><td>31</td><td>32</td><td>2.06</td><td>6.4%</td></tr><tr><td>Sample 2</td><td>254</td><td>260</td><td>260</td><td>224</td><td>249</td><td>14.92</td><td>5.9%</td></tr><tr><td>Sample 3</td><td>445</td><td>507</td><td>470</td><td>490</td><td>478</td><td>23.11</td><td>4.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Tumor necrosis factor ligand superfamily member 8;CD30 ligand;CD30-L;CD153;Tnfsf8;Cd30l, Cd30lg;

Gene Name

Tumor necrosis factor ligand superfamily member 8

Gene ID

21949

UniProt

P32972

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q68-D239

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<6 pg/ml

Function

Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)