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Mouse CCL1/I 309 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0566-carrier-free

Size: 96 Well With Removable Strips

Mouse CCL1/I 309 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>17</td><td>15</td><td>16</td><td>16</td><td>16</td><td>0.7</td><td>4.3%</td></tr><tr><td>Sample 2</td><td>81</td><td>79</td><td>85</td><td>79</td><td>81</td><td>2.44</td><td>3%</td></tr><tr><td>Sample 3</td><td>291</td><td>314</td><td>273</td><td>296</td><td>293</td><td>14.6</td><td>4.9%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-C motif chemokine 1;P500;SIS-epsilon;Small-inducible cytokine A1;T-cell activation protein 3;TCA-3;TCA3;Ccl1;Scya1, Tca3;

Gene Name

C-C motif chemokine 1

Gene ID

20290

UniProt

P10146

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K24-C92

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<1 pg/ml

Function

Cytokine that is chemotactic for neutrophils.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10276 MW

Specificity

Natural and recombinant mouse CCL1

Anticoagulant

heparin or EDTA

Protein Name

C-C motif chemokine 1

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