Human uPA / PLAU / URK ELISA Kit PicoKine®, Carrier-free

Human uPA / PLAU / URK ELISA Kit PicoKine® (96 Tests). Quantitate Human PLAU in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>195</td><td>183</td><td>178</td><td>191</td><td>186</td><td>6.64</td><td>3.5%</td></tr><tr><td>Sample 2</td><td>583</td><td>571</td><td>542</td><td>578</td><td>568</td><td>15.88</td><td>2.7%</td></tr><tr><td>Sample 3</td><td>1373</td><td>1415</td><td>1322</td><td>1294</td><td>1351</td><td>46.55</td><td>3.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Urokinase-type plasminogen activator;U-plasminogen activator;uPA;3.4.21.73;Urokinase-type plasminogen activator long chain A;Urokinase-type plasminogen activator short chain A;Urokinase-type plasminogen activator chain B;PLAU;

Gene Name

Urokinase-type plasminogen activator

Gene ID

5328

UniProt

P00749

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-L431

Tissue Specificity

Expressed in the prostate gland and prostate cancers. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<5 pg/ml

Function

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)