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Human NT-4/NTF4/Neurotrophin-4 ELISA Kit PicoKine®, Carrier-free

Human NT-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human NTF4 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Neurotrophin-4 (NT-4), also known as neurotrophin-5 (NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons (2-4). NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>94</td><td>99</td><td>93</td><td>89</td><td>93</td><td>3.56</td><td>3.8%</td></tr><tr><td>Sample 2</td><td>234</td><td>212</td><td>207</td><td>213</td><td>216</td><td>10.35</td><td>4.7%</td></tr><tr><td>Sample 3</td><td>888</td><td>924</td><td>870</td><td>895</td><td>894</td><td>19.44</td><td>2.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Neurotrophin-4;NT-4;Neurotrophin-5;NT-5;Neutrophic factor 4;NTF4;NTF5;

Gene Name

Neurotrophin-4

Gene ID

4909

UniProt

P34130

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G81-A210

Tissue Specificity

Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Target-derived survival factor for peripheral sensory sympathetic neurons.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22427 MW

Specificity

Natural and recombinant human NT-4

Subcellular Location

Secreted.

Protein Name

Neurotrophin-4

Frequently Asked Questions

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