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Human CCL19/MIP-3 Beta ELISA Kit PicoKine®, Carrier-free

Human CCL19/MIP-3 Beta ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL19 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine?belonging to the CC chemokine family that is also known as?EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta? (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>29</td><td>30</td><td>31</td><td>29</td><td>29</td><td>0.82</td><td>2.8%</td></tr><tr><td>Sample 2</td><td>234</td><td>255</td><td>249</td><td>222</td><td>240</td><td>12.9</td><td>5.3%</td></tr><tr><td>Sample 3</td><td>421</td><td>456</td><td>425</td><td>465</td><td>441</td><td>19.07</td><td>4.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-C motif chemokine ;CCL19 ;hCG_20476 ;

Gene Name

C-C motif chemokine

Gene ID

6363

UniProt

Q6IBD6

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G22-S98

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10993 MW

Specificity

Natural and recombinant human CCL19

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted .

Protein Name

C-C motif chemokine 19

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