Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine®, Carrier-free

Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 3 (CCL3), so known as Macrophage inflammatory protein-1alpha (MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 ly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>18</td><td>19</td><td>17</td><td>17</td><td>17</td><td>0.82</td><td>4.8%</td></tr><tr><td>Sample 2</td><td>74</td><td>69</td><td>76</td><td>69</td><td>72</td><td>3.08</td><td>4.2%</td></tr><tr><td>Sample 3</td><td>240</td><td>225</td><td>228</td><td>207</td><td>225</td><td>11.81</td><td>5.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

C-C motif chemokine 3;Heparin-binding chemotaxis protein;L2G25B;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;SIS-alpha;Small-inducible cytokine A3;TY-5;Ccl3;Mip1a, Scya3;

Gene Name

C-C motif chemokine 3

Gene ID

20302

UniProt

P10855

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-A92

Tissue Specificity

Expressed in lung, spleen, and pancreas.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<10 pg/ml

Function

Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)