Mouse IL-13/Interleukin-13 ELISA Kit PicoKine®, Carrier-free

Mouse IL-13 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases. Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses. IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy. The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>40</td><td>34</td><td>38</td><td>36</td><td>37</td><td>2.23</td><td>6%</td></tr><tr><td>Sample 2</td><td>209</td><td>191</td><td>179</td><td>193</td><td>193</td><td>10.67</td><td>5.5%</td></tr><tr><td>Sample 3</td><td>526</td><td>501</td><td>535</td><td>503</td><td>516</td><td>14.61</td><td>2.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-13;IL-13;T-cell activation protein P600;Il13;Il-13;

Gene Name

Interleukin-13

Gene ID

16163

UniProt

P20109

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S26-F131

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<2 pg/ml

Function

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)