Mouse IL-3 ELISA Kit PicoKine®, Carrier-free

Mouse IL-3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il3 in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant mouse IL-3, consisting of 135 amino acids with the molecular mass of 15KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>16</td><td>17</td><td>16</td><td>17</td><td>16</td><td>0.5</td><td>3.1%</td></tr><tr><td>Sample 2</td><td>101</td><td>102</td><td>103</td><td>94</td><td>100</td><td>3.53</td><td>3.5%</td></tr><tr><td>Sample 3</td><td>278</td><td>254</td><td>257</td><td>231</td><td>255</td><td>16.65</td><td>6.5%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-3;IL-3;Hematopoietic growth factor;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;Il3;Csfmu, Il-3;

Gene Name

Interleukin-3

Gene ID

16187

UniProt

P01586

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A27-C166

Tissue Specificity

Activated T-cells, mast cells, natural killer cells.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants and serum.

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<1 pg/ml

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)