Mouse IGFBP2 ELISA Kit PicoKine®, Carrier-free

Mouse IGFBP2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igfbp2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>160</td><td>171</td><td>174</td><td>173</td><td>169</td><td>5.59</td><td>3.3%</td></tr><tr><td>Sample 2</td><td>970</td><td>1024</td><td>949</td><td>988</td><td>982</td><td>27.52</td><td>2.8%</td></tr><tr><td>Sample 3</td><td>3281</td><td>3102</td><td>2889</td><td>3320</td><td>3148</td><td>170.63</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Insulin-like growth factor-binding protein 2;IBP-2;IGF-binding protein 2;IGFBP-2;mIGFBP-2;Igfbp2;Igfbp-2;

Gene Name

Insulin-like growth factor-binding protein 2

Gene ID

16008

UniProt

P47877

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E35-Q305

Tissue Specificity

Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

125 pg/ml - 8,000 pg/ml

Sensitivity

<10 pg/ml

Function

Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32847 MW

Specificity

Natural and recombinant mouse IGFBP2

Anticoagulant

heparin or EDTA

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 IGFBP N-terminal domain.

Protein Name

Insulin-like growth factor-binding protein 2

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