Mouse IGFBP-1 ELISA Kit PicoKine®, Carrier-free

Mouse IGFBP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Igfbp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>88</td><td>84</td><td>87</td><td>94</td><td>88</td><td>3.63</td><td>4.1%</td></tr><tr><td>Sample 2</td><td>281</td><td>311</td><td>260</td><td>297</td><td>287</td><td>18.97</td><td>6.6%</td></tr><tr><td>Sample 3</td><td>865</td><td>903</td><td>817</td><td>942</td><td>881</td><td>46.24</td><td>5.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Insulin-like growth factor-binding protein 1;IBP-1;IGF-binding protein 1;IGFBP-1;Igfbp1;Igfbp-1;

Gene Name

Insulin-like growth factor-binding protein 1

Gene ID

16006

UniProt

P47876

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-N272

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<3 pg/ml

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration (By similarity). .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)