Mouse Eotaxin-1 ELISA Kit PicoKine®, Carrier-free

Mouse Eotaxin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl11 in cell culture supernatants, serum and plasma (citrate, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>34</td><td>36</td><td>38</td><td>36</td><td>36</td><td>1.41</td><td>3.9%</td></tr><tr><td>Sample 2</td><td>223</td><td>227</td><td>217</td><td>219</td><td>221</td><td>3.84</td><td>1.7%</td></tr><tr><td>Sample 3</td><td>405</td><td>412</td><td>415</td><td>422</td><td>413</td><td>6.1</td><td>1.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Eotaxin;C-C motif chemokine 11;Eosinophil chemotactic protein;Small-inducible cytokine A11;Ccl11;Scya11;

Gene Name

Eotaxin

Gene ID

20292

UniProt

P48298

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H24-P97

Tissue Specificity

Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (citrate, EDTA).

Detection Range

15.6 pg/ml - 1,000 pg/ml

Sensitivity

<1 pg/ml

Function

In response to the presence of allergens, this protein ly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)