Human IGFBP7/Igfbp Rp1 PicoKine® Quick ELISA Kit, Carrier-free

Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors(IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

<h2>Reproducibility</h2><p style="line-height: 0.4;"></p><p>To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.</p><table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1401</td><td>1416</td><td>1369</td><td>1426</td><td>1403</td><td>44.90</td><td>3.2%</td></tr><tr><td>Sample 2</td><td>2986</td><td>3531</td><td>3163</td><td>3154</td><td>3209</td><td>144.38</td><td>4.5%</td></tr><tr><td>Sample 3</td><td>17423</td><td>15436</td><td>16428</td><td>16654</td><td>16485</td><td>560.5</td><td>3.4%</td></tr></table>*number of samples for each test n=16.

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x)|1|12ml Color Developing Reagent (TMB)|1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece