Human TNFRSF18/GITR PicoKine® Quick ELISA Kit, Carrier-free

Human TNFRSF18/GITR PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human TNFRSF18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>135</td><td>136</td><td>127</td><td>144</td><td>135</td><td>6.02</td><td>4.4%</td></tr><tr><td>Sample 2</td><td>705</td><td>808</td><td>735</td><td>671</td><td>729</td><td>50.53</td><td>6.9%</td></tr><tr><td>Sample 3</td><td>1313</td><td>1243</td><td>1354</td><td>1475</td><td>1346</td><td>84.26</td><td>6.2%</td></tr></table>*number of samples for each test n=16.

Synonyms

Tumor necrosis factor receptor superfamily member 18;Activation-inducible TNFR family receptor;Glucocorticoid-induced TNFR-related protein;CD357;TNFRSF18;AITR, GITR;UNQ319/PRO364;

Gene Name

Tumor necrosis factor receptor superfamily member 18

Gene ID

8784

UniProt

Q9Y5U5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q26-E161

Tissue Specificity

Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)