Rat BMP-2 PicoKine® Quick ELISA Kit, Carrier-free

Rat BMP-2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Rat Bmp2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>96</td><td>96</td><td>106</td><td>100</td><td>99</td><td>4.09</td><td>4.1%</td></tr><tr><td>Sample 2</td><td>245</td><td>258</td><td>242</td><td>225</td><td>242</td><td>11.75</td><td>4.8%</td></tr><tr><td>Sample 3</td><td>806</td><td>902</td><td>918</td><td>836</td><td>865</td><td>46.09</td><td>5.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;Bmp2;Bmp-2;

Gene Name

Bone morphogenetic protein 2

Gene ID

29373

UniProt

P49001

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Tissue Specificity

Expressed in femur, calvaria, trachea, lung and ovary.

Applications

ELISA

Standard

10 ng/tube

Sample Type

bone tissue, cell culture supernatants and serum.

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<2 pg/ml

Function

Induces cartilage and bone formation.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)